Loading Events

LLE: Engineering High-Precision, Dynamic Genetic Control Systems for Cellular Reprogramming (Katie Galloway, MIT)

Wed January 31, 2024 @ 11:00 am - 12:00 pm

Katie Galloway, Massachusetts Institute of Technology

Host: Prof. Nicole Weckman

Integrating synthetic circuitry into larger transcriptional networks to mediate predictable cellular behaviors remains a challenge within synthetic biology. In particular, the stochastic nature of transcription makes coordinating expression across multiple genetic elements difficult. Further, delivery of large genetic cargoes limits the efficiency of cellular engineering. Thus, our work is focused on the design of highly-compact genetic tools with a minimal genomic footprint. Co-localization of multiple transcriptional units provides a simple method of compact design. However, co-localization introduces the potential for physical coupling between transcriptional units. To address this challenge, we recently developed a theoretical framework for exploring how DNA supercoils—dynamic structures induced during transcription—influence transcription and gene expression in synthetic and native gene systems. Using this model, we find that DNA supercoiling strongly influences the profile of gene expression and that influence is defined by syntax—the relative orientation and position of genetic elements—and the enclosing boundary conditions. In exploring both synthetic and native gene regulatory networks, we find that supercoiling-mediated feedback changes the behaviors accessible to control and supports (or inhibits) the function of transcriptional networks. Importantly, we have recently confirmed several predictions from this model experimentally and used this model to design circuits with massively improved performance in primary cells. Our results suggest that supercoiling couples behavior between neighboring genes, representing a novel regulatory mechanism. Additionally, our predictions suggest why some circuit designs fail and provide a path to improving transgenic designs. Harnessing the insights from our model will enable enhanced transcriptional control, providing a robust method to tune expression levels, dynamics, and noise needed for the construction of transgenic systems for diverse cell engineering applications including cellular reprogramming.

_____

Katie Galloway is the W. M. Keck Career Development Professor in Biomedical Engineering and Chemical Engineering at Massachusetts Institute of Technology (MIT). Her research focuses on elucidating the fundamental principles of integrating synthetic circuitry to drive cellular behaviors. Her lab focuses on developing integrated gene circuits and elucidating the systems level principles that govern complex cellular behaviors. Her team lever ages synthetic biology to transform how we understand cellular transitions and engineer cellular therapies. Galloway earned a PhD and an MS in Chemical Engineering from the California Institute of Technology (Caltech), and a BS in Chemical Engineering from University of California at Berkeley. She completed her postdoctoral work at the University of Southern California. Her research has been featured in Science, Cell Stem Cell, Cell Systems, and Development. She has won multiple fellowships and awards including the Cellular and Molecular Bioengineering Rising Star, Princeton’s CBE Saville Lecture Award, NIH Maximizing Investigators’ Research Award, the NIH F32, and Caltech’s Everhart Award.

 

View the complete 2023-24 LLE schedule

Event Details

Venue:

Venue

WB116 @ 200 College Street, Toronto ON M5S 3E5

Details

Date:
Wed January 31, 2024
Time:
11:00 am - 12:00 pm
Event Category:
Website:
https://chem-eng.utoronto.ca/event/katie-galloway/

Share Event

Upcoming Events

All
  • All
  • Alumni events
  • Anti-Racism and Cultural Diversity Office events
  • Convocation events
  • Faculty & staff events
  • Info sessions
  • Lectures, seminars and workshops
  • Socials
  • U of T holidays & closures

Spring Convocation Ceremony

Tue June 18, 2024
Congratulations to the graduating class of 2T4 and 2T3+PEY! We look forward to celebrating this important milestone with you at Spring Convocation 2024. Please find full details on the Convocation...

National Indigenous Peoples Day

Fri June 21, 2024
For generations, many Indigenous groups and communities have celebrated their culture and heritage on June 21 or around that time of year because of the significance of the summer solstice...

NSERC Discovery Grant info session 1: Preparing the Notification of Intent

Wed June 26, 2024 @ 12:00 pm - 1:00 pm
The Office of the Vice-Dean, Research is hosting a session for U of T Engineering faculty members on preparing a Discovery Grant application. This session will be held online and...

U of T Presidential Day – University Closed

Fri June 28, 2024
U of T Presidential Day – University Closed