LLE: Enzyme Technology for Enzymatic Synthesis of Human Milk Oligosaccharides (Anne Meyer, Technical University of Denmark)
April 6, 2022 @ 12:00 pm - 1:00 pm
Anne Meyer, Technical University of Denmark
Host: Prof. Emma Master
Enzyme technology now targets using enzymes for catalysing very specific molecular conversions for production of particular compounds. Human milk oligosaccharides (HMOs) are a group of lactose-based carbohydrates which are abundant (5-20 g/L) in human milk. More than 200 different HMO structures have been identified in human milk, but these structures are barely present in bovine milk. HMOs play crucial roles in infant health and development. Hence, a large incentive exists for developing infant formulas that more closely resemble human milk. Natural HMOs consist of up to five different monosaccharides, namely β-D-galactose (Gal), β-D-glucose (Glc), β-D-N-acetylglucosamine (GlcNAc), α-L-fucose (Fuc), and the sialic acid α-D-N-acetylneuraminic acid (Neu5Ac). All HMOs have lactose at the reducing end; lactose can be decorated with Fuc or Neu5Ac and/or further elongated by either β-N-acetyllactosamine or lacto-N-biose units to form linear or branched structures. These elongated parts may also be fucosylated or sialylated. At the Technical University of Denmark, we have embarked on enzymatic synthesis of HMOs, focusing on developing enzymatic trans-sialylation and trans-fucosylation reactions by engineering of protozoan and microbial glycoside hydrolases to work ‘in reverse’, and we have aimed at discovering GH20 β-N-acetylhexosaminidases capable of catalysing backbone elongation of lactose via trans-glycosylation. We are also trying to utilize oxazoline, which is a reaction intermediate in the unique GH20 β-N-acetylhexosaminidase catalyzed reactions. The aim of the lecture is to present the different approaches and at the same time discuss various protein engineering strategies to promote trans-glycosylation.
Anne S. Meyer is Professor of Enzyme Technology at the Technical University of Denmark (DTU) and Head of the Protein Chemistry & Enzyme Technology Division, approx. 75 persons, incl. 7 professor groups, and ~30 PhD students, at Dept. of Biotechnology and Biomedicine (DTU Bioengineering), DTU. She is group leader for the Enzyme Technology group in the Division. Anne holds an MSc from the University of Copenhagen, and an MSc from the University of Reading, UK (1987), and a PhD from DTU (1993). Employed at DTU since 1988 in various positions, and has had two postdoc stays in the USA at University California Davis. She became Full Professor at DTU in 2006 and has headed Center for BioProcess Engineering at DTU until summer 2018, where she assumed her current role as Head of the Protein Chemistry & Enzyme Technology Division at DTU Bioengineering. She has been visiting professor at Dept. Chemical and Biomolecular Engineering, University of Melbourne, Australia from 2017-2020.
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